MicroRNA-122 regulates caspase-8 and promotes the apoptosis of mouse cardiomyocytes
| Autor: | Z.W. Zhang, H. Li, S.S. Chen, Y. Li, Z.Y. Cui, J. Ma |
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| Jazyk: | angličtina |
| Předmět: | |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 50, Iss 2 |
| Druh dokumentu: | article |
| ISSN: | 1414-431X 1414-431x |
| DOI: | 10.1590/1414-431x20165760 |
| Popis: | Cardiomyocyte apoptosis plays key roles in the pathogenesis of heart diseases such as myocardial infarction. MicroRNAs are important regulators of gene expression, which are also involved in the regulation of cardiomyocyte apoptosis. However, cardiomyocyte apoptosis regulated by microRNA (miR)-122 is largely unexplored. The aim of this study focused on the role of miR-122 in cardiomyocyte apoptosis. Cardiomyocytes were isolated from neonatal mice and primarily cultured. MiR-122 mimic and inhibitor were transfected to cardiomyocytes and verified by qRT-PCR. Cell viability and apoptosis post-transfection were assessed by MTT assay and flow cytometry, respectively. Changes in expression of caspase-8 were quantified by qRT-PCR and western blot. Results showed that miR-122 mimic and inhibitor successfully induced changes in miR-122 levels in cultured cardiomyocytes (P |
| Databáze: | Directory of Open Access Journals |
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