Anatomical and histochemical characterization of in vitro haustorium from roots of Castilleja tenuiflora

Autor: G. Salcedo-Morales, A. R. Jiménez-Aparicio, F. Cruz-Sosa, G. Trejo-Tapia
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Biologia Plantarum, Vol 58, Iss 1, Pp 164-168 (2014)
Druh dokumentu: article
ISSN: 1573-8264
DOI: 10.1007/s10535-013-0369-2
Popis: In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 μM catechin, 25 μM vanillin, or 25 μM H2O2. Of the treatments tested, 25 μM vanillin was the strongest inducer of haustoria in C. tenuiflora roots in vitro (up to 3 haustoria per root). Haustorium development occurred laterally and was observable 14 d after inducer application. It was characterized by elongation of the epidermal cells and division of the inner cortical cells which also possessed abundant nuclei. Histochemical analysis using 3,3-diaminobenzidine (DAB) and diphenylboric acid 2-aminoethyl ester (DBPA) indicated that the formation of haustoria was associated with the accumulation of H2O2 and flavonoids.
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