| Autor: |
Laura Cubas-Nuñez, María Duran-Moreno, Jessica Castillo-Villalba, Jorge Fuentes-Maestre, Bonaventura Casanova, José M. García-Verdugo, Sara Gil-Perotín |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Frontiers in Cellular Neuroscience, Vol 11 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1662-5102 |
| DOI: |
10.3389/fncel.2017.00141 |
| Popis: |
In situ RT-PCR detects and amplifies mRNA (cDNA) while obtaining spatial information of gene expression. When the intended use is an ultrastructural analysis of morphology, the procedure may be technically challenging and quality of tissue dramatically altered by proteolytic digestion and extreme astringency and temperature conditions. We describe a low-damaging protocol of in situ RT-PCR combined to conventional electron microscopy that preserves fine morphology, increases sensitivity, and decreases costs and complexity associated to RNA probes. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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