GOBP1 from the Variegated Cutworm Peridroma saucia (Hübner) (Lepidoptera: Noctuidae) Displays High Binding Affinities to the Behavioral Attractant (Z)-3-Hexenyl acetate
| Autor: | Ya-Lan Sun, Jun-Feng Dong, Yue-Qin Song, Shao-Li Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Insects, Vol 12, Iss 10, p 939 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2075-4450 60778741 |
| DOI: | 10.3390/insects12100939 |
| Popis: | The variegated cutworm Peridroma saucia (Hübner) is a worldwide pest that causes serious damage to many crops. To recognize sex pheromones and host plant volatiles, insects depend on olfactory chemoreception involving general odorant-binding proteins (GOBPs). In this study, PsauGOBP1 was cloned from the adult antennae of P. saucia. RT-qPCR and Western-blot analysis showed that PsauGOBP1 was specifically and equally expressed in the adult antennae of both females and males. Fluorescence competitive-binding assays with sex pheromones and host plant volatiles demonstrated that PsauGOBP1 bound to six host plant volatiles: (Z)-3-hexenyl acetate (KD = 4.0 ± 0.1 μM), citral (KD = 5.6 ± 0.4 μM), farnesol (KD = 6.4 ± 0.6 μM), nonanal (KD = 6.8 ± 0.3 μM), (Z)-3-hexen-1-ol (KD = 8.5 ± 0.6 μM), and benzaldehyde (KD = 9.4 ± 0.5 μM). Electroantennogram recordings with the six host plant volatiles indicated that (Z)-3-hexenyl acetate elicited the strongest responses from both male and female antennae. Further bioassays using Y-tube olfactometers showed that (Z)-3-hexenyl acetate was attractive to adult P. saucia of both sexes. These results suggest that PsauGOBP1 might be involved in detecting host plant volatiles and that (Z)-3-hexenyl acetate might serve as a potential attractant for the biological control of P. saucia. |
| Databáze: | Directory of Open Access Journals |
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