From the Field to the Laboratory: Air Pollutant-Induced Genomic Effects in Lung Cells

Autor: William Vizuete, Kenneth G. Sexton, Hang Nguyen, Lisa Smeester, Kjersti Marie Aagaard, Cynthia Shope, Barry Lefer, James H. Flynn, Sergio Alvarez, Mathew H. Erickson, Rebecca C. Fry
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Environmental Health Insights, Vol 9s4 (2015)
Druh dokumentu: article
ISSN: 1178-6302
DOI: 10.4137/EHI.S15656
Popis: Current in vitro studies do not typically assess cellular impacts in relation to real-world atmospheric mixtures of gases. In this study, we set out to examine the feasibility of measuring biological responses at the level of gene expression in human lung cells upon direct exposures to air in the field. This study describes the successful deployment of lung cells in the heavily industrialized Houston Ship Channel. By examining messenger RNA (mRNA) levels from exposed lung cells, we identified changes in genes that play a role as inflammatory responders in the cell. The results show anticipated responses from negative and positive controls, confirming the integrity of the experimental protocol and the successful deployment of the in vitro instrument. Furthermore, exposures to ambient conditions displayed robust changes in gene expression. These results demonstrate a methodology that can produce gas-phase toxicity data in the field.
Databáze: Directory of Open Access Journals
Popis
Abstrakt:Current in vitro studies do not typically assess cellular impacts in relation to real-world atmospheric mixtures of gases. In this study, we set out to examine the feasibility of measuring biological responses at the level of gene expression in human lung cells upon direct exposures to air in the field. This study describes the successful deployment of lung cells in the heavily industrialized Houston Ship Channel. By examining messenger RNA (mRNA) levels from exposed lung cells, we identified changes in genes that play a role as inflammatory responders in the cell. The results show anticipated responses from negative and positive controls, confirming the integrity of the experimental protocol and the successful deployment of the in vitro instrument. Furthermore, exposures to ambient conditions displayed robust changes in gene expression. These results demonstrate a methodology that can produce gas-phase toxicity data in the field.
ISSN:11786302
DOI:10.4137/EHI.S15656