Dynamics of ligninolytic enzyme production in Ganoderma applanatum depending on cultivation type

Autor: Ćilerdžić Jasmina Lj., Vukojević Jelena B., Stajić Mirjana M.
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Zbornik Matice Srpske za Prirodne Nauke, Vol 2011, Iss 120, Pp 327-331 (2011)
Druh dokumentu: article
ISSN: 0352-4906
DOI: 10.2298/ZMSPN1120327C
Popis: Ganoderma applanatum belongs to the group of white-rot fungi, due to a well-developed ligninolytic enzyme system. White-rot fungi have attracted great scientific attention in recent years, especially with respect to their enzymatic potential for the bioremediation of persistent pollutants. Contrary to G. lucidum, which medicinal properties, as well as ligninolytic enzyme system have been extensively studied, enzymatic system of G. applanatum has not been studied yet. Thus, the aim of this study was to analyze the dynamics of laccase, Mn-dependent peroxidase, and versatile peroxidase activity during submerged and solid state cultivation on two selected plant raw materials. Enzyme activity was determined spectrophotometrically after 7, 10 and 14 days of cultivation. The peak of laccase activity (220.14 Ul-1) was noted after 14 days of submerged wheat straw fermentation. Maximum level of Mn-dependent peroxidase (110.91 Ul-1) and versatile peroxidase (116.20 Ul-1) activity was obtained in the medium with oak sawdust after 14 days of submerged cultivation.
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