Dynamics of SARS-CoV2 Infection and Multi-Drug Resistant Bacteria Superinfection in Patients With Assisted Mechanical Ventilation

Autor: Annarita Mazzariol, Anna Benini, Ilaria Unali, Riccardo Nocini, Marcello Smania, Anna Bertoncelli, Francesco De Sanctis, Stefano Ugel, Katia Donadello, Enrico Polati, Davide Gibellini
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Frontiers in Cellular and Infection Microbiology, Vol 11 (2021)
Druh dokumentu: article
ISSN: 2235-2988
DOI: 10.3389/fcimb.2021.683409
Popis: ObjectiveTo investigate the presence of bacteria and fungi in bronchial aspirate (BA) samples from 43 mechanically ventilated patients with severe COVID-19 disease.MethodsDetection of SARS-CoV-2 was performed using Allplex 2019-nCoV assay kits. Isolation and characterisation of bacteria and fungi were carried out in BA specimens treated with 1X dithiothreitol 1% for 30 min at room temperature, using standard culture procedures.ResultsBacterial and/or fungal superinfection was detected in 25 out of 43 mechanically ventilated patients, generally after 7 days of hospitalisation in an intensive care unit (ICU). Microbial colonisation (colony forming units (CFU)
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