Sperm filtration as an alternative technique for seminal plasma separation in boars

Autor: Carlos Augusto Alves Melanda, Ana Beatriz Marques de Almeida, Myrian Megumy Tsunokawa Hidalgo, Anne Kemmer Souza, Luiz Guilherme Corsi Trautwein, Jamile Haddad Neta, Évelyn Rangel dos Santos, Antonio Cezar de Oliveira Dearo, Maria Isabel Mello Martins
Jazyk: English<br />Portuguese
Rok vydání: 2021
Předmět:
Zdroj: Semina: Ciências Agrárias, Vol 42, Iss 4 (2021)
Druh dokumentu: article
ISSN: 1679-0359
1676-546X
DOI: 10.5433/1679-0359.2021v42n4p2439
Popis: The present study aimed to evaluate the filtration for separating seminal plasma of boars’ ejaculate by means of sperm viability and the occurrence of hyperactivation and lipid peroxidation in fresh semen and after cooling for up to 96 hours. The ejaculate of eight healthy boars of different breeds was collected and the gelatinous portion was separated and discarded. In the laboratory, the semen was fractioned into three aliquots (groups G1, G2 and G3) as follows: G1: semen with plasma diluted in BTS (TOTAL BTS); G2: semen centrifuged at 600G/10’ (BTS CEN); and G3: semen filtered with the Sperm-filter® following dilution of the retained cells with BTS (BTS FIL). The analyses were performed at three moments: with fresh samples (D0) and after 48 (D2) and 96 hours (D4) of cooling at 17ºC. The kinetic evaluation was performed using the CASA system, which provided data for the classification of sperm hyperactivity. For lipid stress analysis, the TBARS (thiobarbituric acid reactive substance) test was performed. The variance analysis test was conducted to compare the results between the groups and moments analyzed. The results showed better total motility values (%) for G1 at D0 (67.9, P= 0.001), D2 (36.6, P= 0.004) and D4 (26.1, P= 0.003). The occurrence of hyperactivity was observed in G2 and G3 at moments D2 and D4. In addition, TBARS showed higher peroxidation levels for G1 at D0 (8.1 mM MDA/ml, P= 0.01), D2 (7.4 mM MDA/ml, P= 0.02), and D4 (6.41mMol MDA/ml, P= 0.008) when compared to G2 and G3. Since the filtration method did not demonstrate any damage to the sperm viability, the study concluded that sperm filtration is an accessible and valid tool to replace centrifugation.
Databáze: Directory of Open Access Journals
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