| Autor: |
Catherine M.C. Volgenant, Michel A. Hoogenkamp, Mark J. Buijs, Egija Zaura, Jacob (Bob) M. ten Cate, Monique H. van der Veen |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Journal of Oral Microbiology, Vol 8, Iss 0, Pp 1-9 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2000-2297 |
| DOI: |
10.3402/jom.v8.30346 |
| Popis: |
Background: Some dental plaque fluoresces red. The factors involved in this fluorescence are yet unknown. Objective: The aim of this study was to assess systematically the effect of age, thickness, and cariogenicity on the extent of red fluorescence produced by in vitro microcosm biofilms. Design: The effects of biofilm age and thickness on red fluorescence were tested in a constant depth film fermentor (CDFF) by growing biofilms of variable thicknesses that received a constant supply of defined mucin medium (DMM) and eight pulses of sucrose/day. The influence of cariogenicity on red fluorescence was tested by growing biofilm on dentin disks receiving DMM, supplemented with three or eight pulses of sucrose/day. The biofilms were analyzed at different time points after inoculation, up to 24 days. Emission spectra were measured using a fluorescence spectrophotometer (λexc405 nm) and the biofilms were photographed with a fluorescence camera. The composition of the biofilms was assessed using 454-pyrosequecing of the 16S rDNA gene. Results: From day 7 onward, the biofilms emitted increasing intensities of red fluorescence as evidenced by the combined red fluorescence peaks. The red fluorescence intensity correlated with biofilm thickness but not in a linear way. Biofilm fluorescence also correlated with the imposed cariogenicity, evidenced by the induced dentin mineral loss. Increasing the biofilm age or increasing the sucrose pulsing frequency led to a shift in the microbial composition. These shifts in composition were accompanied by an increase in red fluorescence. Conclusions: The current study shows that a thicker, older, or more cariogenic biofilm results in a higher intensity of red fluorescence. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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