| Autor: |
Bonin Michael, Wild David L, Rand David A, Dijkhuizen Lubbert, Jansen Ritsert C, Challis Gregory L, Legaie Roxane, Gaze William H, Iqbal Mudassar, Thomas Louise, Nentwich Merle, Rodríguez-García Antonio, Juarez-Hermosillo Miguel A, Morrissey Edward R, Omara Walid AM, Moore Jonathan, Merlo Maria E, Alam Mohammad T, Sletta Håvard, Jakobsen Øyvind M, Wentzel Alexander, Bruheim Per, Herbig Alexander, Battke Florian, Nieselt Kay, Reuther Jens, Wohlleben Wolfgang, Smith Margaret CM, Burroughs Nigel J, Martín Juan F, Hodgson David A, Takano Eriko, Breitling Rainer, Ellingsen Trond E, Wellington Elizabeth MH |
| Jazyk: |
angličtina |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
BMC Genomics, Vol 11, Iss 1, p 10 (2010) |
| Druh dokumentu: |
article |
| ISSN: |
1471-2164 |
| DOI: |
10.1186/1471-2164-11-10 |
| Popis: |
Abstract Background During the lifetime of a fermenter culture, the soil bacterium S. coelicolor undergoes a major metabolic switch from exponential growth to antibiotic production. We have studied gene expression patterns during this switch, using a specifically designed Affymetrix genechip and a high-resolution time-series of fermenter-grown samples. Results Surprisingly, we find that the metabolic switch actually consists of multiple finely orchestrated switching events. Strongly coherent clusters of genes show drastic changes in gene expression already many hours before the classically defined transition phase where the switch from primary to secondary metabolism was expected. The main switch in gene expression takes only 2 hours, and changes in antibiotic biosynthesis genes are delayed relative to the metabolic rearrangements. Furthermore, global variation in morphogenesis genes indicates an involvement of cell differentiation pathways in the decision phase leading up to the commitment to antibiotic biosynthesis. Conclusions Our study provides the first detailed insights into the complex sequence of early regulatory events during and preceding the major metabolic switch in S. coelicolor, which will form the starting point for future attempts at engineering antibiotic production in a biotechnological setting. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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