Autor: |
Sohini Banerjee, Uma Nahar, Divya Dahiya, Soham Mukherjee, Pranab Dey, Rijuneeta Gupta, Bishan Radotra, Naresh Sachdeva, Ashwani Sood, Sanjay Kumar Bhadada, Anil Bhansali |
Jazyk: |
angličtina |
Rok vydání: |
2022 |
Předmět: |
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Zdroj: |
Frontiers in Endocrinology, Vol 13 (2022) |
Druh dokumentu: |
article |
ISSN: |
1664-2392 |
DOI: |
10.3389/fendo.2022.931647 |
Popis: |
BackgroundLymphocytic thyroiditis (LT) is frequently seen in the tumor microenvironment (TME) of papillary thyroid carcinomas (PTCs). However, the characteristic of these tumor-infiltrating lymphocytes (TILs) is not well understood.ObjectiveWe aim to define the TME of PTC cases by characterizing the TILs.DesignThis is a cross-sectional observational study.PatientsWe enrolled 29 PTC (23 having concurrent LT), 14 LT, and 13 hyperplastic nodules with LT (HN) patients from January 2016 to December 2020.MeasurementsImmunohistochemical (IHC) expression of CD8, FoxP3, PD-1, and PD-L1 was studied in PTC with LT and compared with HN. PD-1 and PD-L1 expression was correlated at the mRNA level by quantitative real-time PCR. Immunophenotyping of TILs was done in FNAC samples of PTC and LT by flow cytometry.ResultsIHC revealed the presence of CD8+ cytotoxic T lymphocytes (CTLs) and FoxP3+ T regulatory cells (Tregs) in 83% and 52% of PTC with LT cases, respectively. Flow cytometric analysis of the PTC samples revealed a significant abundance of CTL compared with Treg and a higher CTL with lower Treg counts compared with LT. On IHC, PD-1 positivity was noted in 56.5% of PTC with LT cases, while intermediate PD-L1 positivity was found in 70% of the cases. There was a significant upregulation of PD-1 mRNA in PTC with LT. A significant correlation was noted with PD-L1 expression with lymph node metastasis and presence of Treg cells.ConclusionsIncreased expression of PD-1 and PD-L1 in the TME of PTC may provide a potential molecular mechanism for tumor survival despite the predominance of CTLs, possibly through their inactivation or exhaustion. |
Databáze: |
Directory of Open Access Journals |
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