Detection of Fasciola hepatica using nested-PCR in the slaughterhouses of Alborz, Iran
| Autor: | A. Rezaee Kalvani, L. Abdolmohammadi Khiav, S. R. Hosseini |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Bulgarian Journal of Veterinary Medicine, Vol 27, Iss 4, Pp 639-645 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1311-1477 1313-3543 |
| DOI: | 10.15547/bjvm.2023-0035 |
| Popis: | Fasciola hepatica is a parasitic liver trematode that causes fasciolosis in humans and dairy animals. Traditional detection of infection is based on a microscopic examination with low sensitivity. There-fore, this study was carried out to develop an accurate and rapid method to detect F. hepatica in Al-borz province. In this cross-sectional study, 386 samples were collected from livers of cattle and sheep in three slaughterhouses of Alborz. DNA was extracted, and nested-PCR was carried out based on the F. hepatica ITS-2 sequence. DNA amplification showed first and second PCR products with expected size of 336 and 208 bp respectively, as unique for F. hepatica. The results showed no cross-reaction with the negative control. Thirty-five liver samples were positive for this parasitic infection. Totally, the prevalence of F. hepatica in the slaughterhouses of Alborz, Iran was 9.07%. This is the first study of the molecular detection of F. hepatica using nested-PCR in Alborz. It is a sensitive and rapid method that will help evaluate the prevalence rate of F. hepatica infection. |
| Databáze: | Directory of Open Access Journals |
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