Autor: |
Vesna Župunski, Radivoje Jevtić, Mirjana Lalošević, Sanja Mikić, Branka Orbović |
Jazyk: |
angličtina |
Rok vydání: |
2021 |
Předmět: |
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Zdroj: |
Agronomy, Vol 11, Iss 4, p 778 (2021) |
Druh dokumentu: |
article |
ISSN: |
2073-4395 |
DOI: |
10.3390/agronomy11040778 |
Popis: |
Monitoring changes in the prevalence of Fusarium species and toxin production is an important tool for the integrated control of Fusarium head blight (FHB). However, methods for the high-throughput screening of Fusarium populations have been developed using isolates with limited geographic origins. In this study, we used species- and trichothecene-specific primers to monitor the F. graminearum species complex (FGSC) originating from Serbia. We also tested the applicability of the primers to the surveillance of FHB. We analyzed two hundred and ten isolates collected from thirty two locations and five winter wheat varieties over a three-year period. Using multiple correspondence analysis (MCA), we investigated associations between Fusarium-damaged kernels (FDK) and location, variety, members of the FGSC, and their predisposition for mycotoxin production. The results revealed that the species-specific primers were not specific for 11% of the F. graminearum population. The primer sets were 98.5%, 95.2%, and 92.4% effective in the multilocus genotyping of Tri7, Tri3, and Tri5 genes, respectively. We found that individual wheat varieties were associated with isolates that could not be characterized using species- and trichothecene-specific primers. Alternaria spp. had a significant influence (p < 0.001) on grain infection with F. graminearum, indicating the necessity to further investigate its impact on the pathogenesis of the F. graminearum clade. |
Databáze: |
Directory of Open Access Journals |
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