Anticancer efficacy of perillyl alcohol-bearing PLGA microparticles

Autor: Farazuddin M, Sharma B, Khan AA, Joshi B, Owais M
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Zdroj: International Journal of Nanomedicine, Vol 2012, Iss default, Pp 35-47 (2012)
Druh dokumentu: article
ISSN: 1176-9114
1178-2013
75728354
Popis: Mohammad Farazuddin1, Bhawna Sharma2, Aijaz Ahmed Khan3, Beenu Joshi2, Mohammad Owais11Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, Uttar Pradesh, India; 2Immunology Division, NJIL and other Mycobacterial diseases, Agra-282001, Uttar Pradesh, India; 3Department of Anatomy, JN Medical college, Aligarh Muslim University, Aligarh-202002, Uttar Pradesh, IndiaAbstract: In the present study, a novel poly-lactic glycolic acid (PLGA)-based microparticle formulation of perillyl alcohol (POH) was prepared and characterized. Further, its efficacy was evaluated against di-methyl benzo anthracene-induced skin papilloma in Swiss albino mice. The characterization studies showed that POH-bearing PLGA microparticles were of the size 768 ± 215 nm with a ζ-potential value of -7.56 ± 0.88 mV. The entrapment efficiency of the active drug in particles was 42.4% ± 3.5%. POH-bearing PLGA microparticles were stable and released entrapped drug gradually over an extended time period. The in vitro efficacy of POH-bearing PLGA microparticles was evaluated by examining their differential cytotoxicity and assessing their ability to inhibit epidermoid carcinoma cell line (A253). The POH-based microparticles when administered to tumor-bearing animals caused greater tumor regression and increased survival rate (~80%) as compared with the group receiving free form of POH (survival rate 40%). The superiority of POH-PLGA microparticles over free form of POH was further evident from their ability to modulate apoptosis-regulating factors.Keywords: poly-lactic glycolic acid, epidermoid cancer cells, skin papilloma, anticancer efficacy
Databáze: Directory of Open Access Journals
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