Effects of Gamma Irradiation on Changes in Chemical Composition and Antioxidant Activity of Euphorbia maculata Callus
| Autor: | Gyeong Han Jeong, Shubhpreet Kaur, Youngchul Yoo, Young Bae Ryu, Seo Jun Lee, Kwang-Woo Jung, Moon-Soo Chung, Hyoung-Woo Bai, Jin-Hong Kim, Sungbeom Lee, Tae Hoon Kim, Byung Yeoup Chung, Seung Sik Lee |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Plants, Vol 13, Iss 16, p 2306 (2024) |
| Druh dokumentu: | article |
| ISSN: | 13162306 2223-7747 |
| DOI: | 10.3390/plants13162306 |
| Popis: | In this study, we investigated the effects of gamma irradiation on the antioxidant activity and metabolite profiles of Euphorbia maculata calli (PC3012). Gamma irradiation at various doses (0, 0.05, 0.5, and 10 kGy) significantly enhanced the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS+) radical scavenging activities of the callus extracts of PC3012 in a dose-dependent manner. High-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-Q-TOF/MS) analyses revealed that irradiation increased the lysophospholipid content, although no new antioxidant compounds were formed. Furthermore, a PLS-DA analysis revealed evident metabolic differences between non-irradiated and irradiated samples, which were further verified by statistical validation. These findings suggest that gamma irradiation induces specific biochemical modifications that enhance the bioactive properties of PC3012 calli. This technology exhibits potential for utilization in the natural product and food sectors, particularly in the development of functional foods and nutraceuticals with improved health benefits. |
| Databáze: | Directory of Open Access Journals |
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