Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNA
Autor: | Jiana Jiang, Xianming Kong, Yuexia Xie, Hanbing Zou, Qianyun Tang, Ding Ma, Xue Zhao, Xiaozhen He, Anyue Xia, Peifeng Liu |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: | |
Zdroj: | Nanoscale Research Letters, Vol 14, Iss 1, Pp 1-10 (2019) |
Druh dokumentu: | article |
ISSN: | 1931-7573 1556-276X |
DOI: | 10.1186/s11671-019-3032-9 |
Popis: | Abstract Unmethylated CpG oligodeoxynucleotides are potent immunostimulatory motifs in activating both innate and acquired immune system by inducing Th1 type antigen-specific T cell responses, but their instability in serum greatly influences their immunostimulant efficiency. Here, we constructed a novel immuno-DNA nanohydrogels consisting of tandem repeat sequences of CpG units named CpG-MCA nanohydrogels through multi-primed chain amplification. CpG-MCA nanohydrogels were proved to resist degradation and increase the proliferation and migration of murine macrophage-like RAW264.7 cells. Furthermore, CpG-MCA nanohydrogels effectively induced high expression of tumor necrosis factor-α and interleukin-6, and remarkably inhibited the proliferation of U251 cells, suggesting that CpG-MCA nanohydrogels are expected to be employed as the potent anti-cancer immunostimulant. |
Databáze: | Directory of Open Access Journals |
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