| Autor: |
Marián Masár, Jasna Hradski, Eva Vargová, Adriána Miškovčíková, Peter Božek, Juraj Ševčík, Roman Szucs |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Separations, Vol 7, Iss 4, p 72 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2297-8739 |
| DOI: |
10.3390/separations7040072 |
| Popis: |
This paper presents a novel miniaturized analytical method for the determination of carminic acid, a natural red food dye, in complex food and pharmaceutical matrices by microchip electrophoresis (MCE) with photometric detection. MCE has become a very attractive microscale separation technique because it offers high-speed, high-throughput, small sample injection volume and low reagents consumption. Fast determination of carminic acid in less than 5 min was achieved on a poly(methyl methacrylate) microchip in anionic separation mode at pH 6. Photometric detector based on light-emitting diode technology was set to a wavelength of 490 nm. Using a sample injection volume of 900 nL, a limit of detection of 69 nmol L−1 was achieved. A wide linear dynamic range over four orders of magnitude (from nmol L−1 to mmol L−1) was observed for peak area. Developed method provided favorable intra- and inter-day repeatability of the migration time (up to 2.5% RSD), as well as the repeatability of the peak area (less than 1.9% RSD), regardless of the sample type. The content of carminic acid was determined in various foodstuffs and pharmaceuticals, such as candies, saffron, non-alcoholic drink, and sore throat lozenges with good recoveries (92.5–104.0%). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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