Rosiglitazone and Pioglitazone Alter Aromatase Kinetic Properties in Human Granulosa Cells

Autor:	Takako Araki, Miroslava Varadinova, Michael Goldman, Zev Rosenwaks, Leonid Poretsky, Donna Seto-Young
Jazyk:	angličtina
Rok vydání:	2011
Předmět:	Biology (General) QH301-705.5
Zdroj:	PPAR Research, Vol 2011 (2011)
Druh dokumentu:	article
ISSN:	1687-4757 1687-4765
DOI:	10.1155/2011/926438
Popis:	We have previously reported that, in human granulosa cells, thiazolidinediones rosiglitazone and pioglitazone inhibit estrogen synthesis by interfering with androgen binding to aromatase, without an effect on aromatase mRNA or protein expression. In the current paper, we explore the effects of rosiglitazone and pioglitazone on the aromatase enzyme kinetic properties in human granulosa cells. The cells were incubated with various concentrations of testosterone or androstenedione, with or without rosiglitazone or pioglitazone. Estradiol and estrone concentrations in the conditioned tissue culture medium were measured by radioimmunoassay or immunosorbent assay. When testosterone was used as substrate, rosiglitazone or pioglitazone inhibited the Vmax by 35% (P
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/fcda5c4967524f399c1df343b08d6097 Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF