Kindred DNA Amplification from Two Distinct Populations of cDNA Fragments
| Autor: | Ulo Puurand, Lumme Kadaja, Enn K. Seppet |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 34, Iss 5, Pp 994-1000 (2003) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/03345st06 |
| Popis: | Kindred DNA amplification is a novel and cost-effective method developed to isolate common cDNA fragments between two distinct cDNA populations. Unlike subtractive hybridization, which discards common sequences, kindred DNA amplification isolates and amplifies these sequences within a single hybridization procedure. The utility of this method is demonstrated by cloning the genes in common between two different but metabolically homologous muscles, murine ventricular myocardium and soleus. The reliability of kindred DNA amplification was confirmed by Southern hybridization. |
| Databáze: | Directory of Open Access Journals |
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