Sterilization methods for humam teeth

Autor: Rafael Jordão Storino Vaz MONTEIRO, Bárbara Daphini Matos SILVA, Lorena Esteve SILVEIRA, Maria Eugênia ALVAREZ-LEITE, Flavio Ricardo MANZI, Claudia Valéria de Sousa Rezende PENIDO
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: RGO: Revista Gaúcha de Odontologia, Vol 68 (2020)
Druh dokumentu: article
ISSN: 1981-8637
DOI: 10.1590/1981-863720200003520190072
Popis: ABSTRACT Appropriate sterilization methods for dental substrate are necessary so that extracted teeth can be used in laboratory activities, since potentially pathogenic microorganisms may be present even if teeth have been cleaned. Objective Evaluating the sterilization process of human teeth performed by microwave irradiation and autoclave sterilization. Methods Fifty human premolar teeth were sectioned in two portions and distributed in two test groups: G1 - autoclave sterilization for 20 minutes, 1Kgf/cm2, at 127ºC; G2 - Microwave irradiation in a recipient containing 100ml of distilled water, 900W for 5 minutes. Before and after the decontamination process, all teeth were immersed in Brain Heart Infusion (BHI) broth to verify their contamination (positive control) and decontamination potential respectively. Results The statistical analysis of the Friedman test (p
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