Molecular Genotyping of Giardia duodenalis in Humans in the Yazd County, Central of Iran
| Autor: | Elaheh Hezardastan, Ali Fattahi Bafghi, Gilda Eslami, Mahmoud Vakili |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Iranian Journal of Parasitology, Vol 19, Iss 1 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1735-7020 2008-238X |
| Popis: | Background: To investigate Molecular Genotyping of Giardia duodenalis in Humans in the Yazd County, Central of Iran. Methods: Total of 35 fecal samples were collected from patients referred to Yazd central laboratory from February to July 2022. All the samples were included in this study after microscopic observation of G duodenalis. DNA samples were extracted using related kit and were analyzed by Nano Drop. The molecular assessment was carried out using semi-nested PCR using the target gene of gdh. All amplified samples were sequenced using Sanger method. The sequences were analyzed by BLAST for assemblage identification. Results: Out of 35 samples, 24 (68.57%) and 11 (31.43%) were male and female, respectively. All included samples were amplified using the specific gdh primer pair. The molecular analysis showed 17 isolates (48.57%) as assemblage BIV, 8 isolates (22.86%) as assemblage BIII, 6 isolates (17.14%) as assemblage AII and 4 isolates (11.43%) as assemblage AIII (p |
| Databáze: | Directory of Open Access Journals |
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