| Autor: |
Kyoko Ochiai, Hiroki Shima, Tsuyoshi Ikura, Marissa C. Franke, Evelyn P. Sievert, Roger Sciammas, Kazuhiko Igarashi |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
STAR Protocols, Vol 2, Iss 3, Pp 100633- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2021.100633 |
| Popis: |
Summary: Molecular-level understanding of plasma cell (PC) differentiation has been modeled using lipopolysaccharide (LPS) stimulation in vitro. However, this system does not involve the B-cell receptor (BCR)—a critical component of B cell biology. Here, we present a protocol for in vitro PC differentiation system dependent on BCR signaling that easily scales up for cell number-demanding applications, including protein complex purification. We describe how to set up this system and detail applications for endogenous complex purification of chromatin-associated proteins.For further details on the use and execution of this protocol, please refer to Sciammas et al. (2011) and Ochiai et al. (2018, 2020). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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