Effect of Supplementation of Trehalose, Glycerol on Conventional Freezing and Vitrification of Boar Sperm
| Autor: | Sun-Ho Choi, Mi-Jin Lee, Kyung-Mi Lee, Soo-Jin Sa, Hyun-Jong Kim, Hyun-Ju Jin, Yong-Sup Song, Jun-Cheol Park |
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| Jazyk: | English<br />Korean |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Journal of Animal Reproduction and Biotechnology, Vol 29, Iss 4, Pp 397-401 (2014) |
| Druh dokumentu: | article |
| ISSN: | 2671-4639 2671-4663 |
| DOI: | 10.12750/JET.2014.29.4.397 |
| Popis: | The boar sperm has more lipid droplets and specialty of seminal plasma compared with other species, causing difficulties of freezing sperm and decreases for the utilization of frozen semen into the artificial insemination. However, several studies reported significant results for the recovery of sperm motility and reproductive by addition of cryopro- tectants and seminal plasma after thawing. This study was designed to investigate the effects of supplementation of trehalose or glycerol in the LEY (lactose and egg yolk in BTS) solution for the conventional freezing and vitrification process. Two boars aged 16 months were used to collect semen for 2 times in a week. The samples were allotted to 3 freezing solutions (LEY + glycerol 10.5% + OEP 1.5%, LEY + trehalose 1M + OEP 1.5%, and sucrose 1.5M + trehalose 1 M + OEP 1.5%) after centrifugation at 800 g for 10 minutes. Semen was equilibrated in freezing solutions for 10 minutes and injected into plastic straws with 2∼3 air bubbles to minimize freezing damages. Vitrification was performed to locate sperm in 5 cm above LN2 for 5 minutes, and the conventional freezing was conducted with an automatic freezer. Motility and survival rates were measured by CASA (Computer assisted sperm an alyzing system) and FITC (Fluorescein isothiocyanate), respectively after thawing semen at 50℃ for 12 seconds. The results were analyzed by ANOVA with STATVIEW statistical program. The vitrificatioin solution (LEY + 10.5% glycerol + 1.5% OEP) presented higher motility (20.9%) than other solutions while the solution (LEY + 1M trehalose + 1.5% OEP) showed the lowest (motility:5.2%). However, survival rates of vitrified sperms detected by FITC showed 1~4% live sperms in almost of dead sperms at all vitrification solutions’ groups, but survival rate of freezing solution of LEY + 1M trehalose + 1.5% OEP LEY and LEY + 10.5% glycerol + 1.5% OEP were showed 49%, and 79%, respectively. There were differences (P |
| Databáze: | Directory of Open Access Journals |
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