Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus

Autor: Hsu Yau-Heiu, Su Shiaw-Hwa, Chou Yuan-Lin, Tseng Yang-Hao, Hsu Hsiu-Ting, Chang Ban-Yang
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Zdroj: Virology Journal, Vol 6, Iss 1, p 50 (2009)
Druh dokumentu: article
ISSN: 1743-422X
DOI: 10.1186/1743-422X-6-50
Popis: Abstract The triple-gene-block protein 2 (TGBp2) of Bamboo mosaic virus (BaMV) is a transmembrane protein which was proposed to be involved in viral RNA binding during virus transport. Here, we report on the RNA-binding properties of TGBp2. Using tyrosine fluorescence spectroscopy and UV-crosslinking assays, the TGBp2 solubilized with Triton X-100 was found to interact with viral RNA in a non-specific manner. These results raise the possibility that TGBp2 facilitates intracellular delivery of viral RNA through non-specific protein-RNA interaction.
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