Role Of Vascular Endothelial Growth Factor And Human Umbilical Vein Endothelial Cells In Designing An In Vitro Vascular-Muscle Cellular Model Using Adipose-Derived Stem Cells
| Autor: | Abbas Heidari-Moghadam, Vahid Bayati, Mahmoud Orazizadeh, Mohammad Rashno |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Cell Journal, Vol 22, Iss suppl 1, Pp 19-28 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2228-5806 2228-5814 |
| DOI: | 10.22074/cellj.2020.7034 |
| Popis: | Objective Researchers have been interested in the creation of a favorable cellular model for use in vascular-muscle tissue engineering. The main objective of this study is to determine the myogenic effects of vascular endothelial growth factor (VEGF) and human umbilical vein endothelial cells (HUVECs) on adipose-derived stem cells (ADSCs) to achieve an in vitro vascular-muscle cellular model. Materials And Methods The present experimental research was conducted on two primary groups, namely ADSCs monoculture and ADSCs/HUVECs co-culture that were divided into control, horse serum (HS), and HS/VEGF differentiation subgroups. HUVECs were co-cultured by ADSC in a ratio of 1:1. The myogenic differentiation was evaluated using the reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence in different experimental groups. The interaction between ADSCs and HUVECs, as well as the role of ADSCs conditional medium, was investigated for endothelial tube formation assay. Results Immunofluorescence staining indicated that Tropomyosin was positive in ADSCs and ADSCs and HUVECs co-culture groups on HS and HS/VEGF culture medium. Furthermore, the MyHC2 gene expression significantly increased in HS and HS/VEGF groups in comparison with the control group (P |
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