Autor: |
Mingxue Gu, Meiqin Hu, Taylor Minckley, Prateeksunder Pinchi, Haoxing Xu, Yan Qin, Wanlu Du |
Jazyk: |
angličtina |
Rok vydání: |
2022 |
Předmět: |
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Zdroj: |
STAR Protocols, Vol 3, Iss 2, Pp 101453- (2022) |
Druh dokumentu: |
article |
ISSN: |
2666-1667 |
DOI: |
10.1016/j.xpro.2022.101453 |
Popis: |
Summary: Intracellular vesicles such as lysosomes contain micromolar to millimolar concentrations of Zn2+, and disturbing lysosomal Zn2+ homeostasis via lysosomal Zn2+ release leads to mitochondria damage and consequent lytic cell death. Methods have been developed to image cellular Zn2+ dynamics. Here, we present a protocol using GZnP3, a genetically encoded fluorescent Zn2+ indicator, to assess lysosomal Zn2+ release in cultured cells by fluorescence microscopy imaging.For complete details on the use and execution of this protocol, please refer to Du et al. (2021) or Minckley et al. (2019). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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