| Autor: |
Yee Ling Lau, Ilyiana Ismail, Nur Izati Mustapa, Meng Yee Lai, Tuan Suhaila Tuan Soh, Afifah Hassan, Kalaiarasu M. Peariasamy, Yee Leng Lee, Yoong Min Chong, I-Ching Sam, Pik Pin Goh |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
PeerJ, Vol 8, p e9278 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2167-8359 |
| DOI: |
10.7717/peerj.9278 |
| Popis: |
Background Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid. Methods A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. Results This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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