Broad-Host-Range Mobilizable Suicide Vectors for Promoter Trapping in Gram-Negative Bacteria
| Autor: | Mounir Izallalen, Roger C. Levesque, Xavier Perret, William J. Broughton, Hani Antoun |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 33, Iss 5, Pp 1038-1043 (2002) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/02335st04 |
| Popis: | Here we report the construction of three different vectors for the identification of bacterial genes induced in vitro and/or in vivo. These plasmids contain kanamycin, gentamicin, or tetracycline resistance genes as selectable markers. A promoterless cat and an improved GFP (mut3-gfp) can be used to follow the induction of gene expression by measuring chloramphenicol resistance and fluorescence, respectively. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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