| Autor: |
Jiao LEI, Qiju SHAO, Xin XIAO, Zhirong LI, Jinling HONG, Sen WANG, Rongxiang CHEN |
| Jazyk: |
čínština |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Shipin gongye ke-ji, Vol 45, Iss 4, Pp 221-228 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1002-0306 |
| DOI: |
10.13386/j.issn1002-0306.2023030263 |
| Popis: |
Objective: To establish a high performance liquid chromatography-electrochemical detection (HPLC-ECD) method for the determination of polyphenolic compounds in the leaves of Houttuynia cordata and investigate the contribution of different compounds to the antioxidant activity by the correlation analysis. Methods: The leaves of Houttuynia cordata were extracted by ultrasonic extraction with 80% (v/v) methanol, and the content of 12 polyphenolic compounds was determined by the established HPLC-ECD method. The total phenolics content of Houttuynia cordata leaves was determined by the Folin-ciocalteu method. The antioxidant activities were evaluated by radical scavenging ability and ferric reducing antioxidant power (FRAP). Cluster heat map and gray correlation analysis were used for multivariate statistical analysis. Results: The established HPLC-ECD method showed good linearity, precision, repeatability and stability, with an average recovery of 93.25%~103.97% and RSD value of 1.99%~4.47%. The total phenolic content of Houttuynia cordata leaves from different sources ranged from 5.98~52.86 mg GAE/g, which was significantly correlated with ABTS+, DPPH radical scavenging ability and ferric ion reduction ability (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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