Protoplast Isolation in Lupin Lupinus spp
| Autor: | Necmi Beşer, Andrew Wetten |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: | |
| Zdroj: | Journal of Agricultural Sciences, Vol 02, Iss 02, Pp 73-79 (1996) |
| Druh dokumentu: | article |
| ISSN: | 1300-7580 2148-9297 |
| DOI: | 10.1501/Tarimbil_0000000674 |
| Popis: | The aim of this wprk was to find out a method for isolation of+ protoplasts from various lupin Lupinus spp species. Al 10 ml I , CaCl2. 2H20 0.103 g I , Cellulase Onozuka R-10 1.5 %, Macerozyme R-10 0.1%, mannitol 8.96 % and BSA 0.1% have been found to be a suitable enzyme mixt yre for isolating protoplasts from the leaves of glasshouse-grown lupin species. Digestion in the dark in the refrigerator C for 14-15 hours followed by incubation at room temperature for 30- 60 minutes and then shaking at 70 rpm at 25 C for 30 minutes released a high quality and number of protoplasts in all lupin species examined. |
| Databáze: | Directory of Open Access Journals |
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