Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide

Autor: Po-Jung Jimmy Huang, Juewen Liu
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: Nanomaterials, Vol 3, Iss 2, Pp 221-228 (2013)
Druh dokumentu: article
ISSN: 2079-4991
DOI: 10.3390/nano3020221
Popis: Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixing and centrifugation operation. The effect of DNA length and salt on adsorption selectivity has been characterized and its application in DNA melting curve measurement has been demonstrated.
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