Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm
| Autor: | T.F. Collares, V.F. Campos, G. Urtiaga, P.M.M. Leon, M.G. Amaral, C.P. Hartleben, A.J. McBride, O.A. Dellagostin, J.C. Deschamps, F.K. Seixas, T. Collares |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Animal, Vol 7, Iss 5, Pp 778-783 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1751-7311 17517311 |
| DOI: | 10.1017/S1751731112002157 |
| Popis: | Erythropoietin (EPO) gene therapy can be used for several purposes; however, its effects on reproductive performance are unknown. The aim of this study was to evaluate the toxicological effects of non-viral (EPO) gene transfer on sperm motility, viability, morphology and concentration. Rabbit EPO cDNA was cloned into a pTarget mammalian expression vector. Rabbits were administered with: (1) pTarget/EPO vector, (2) recombinant human EPO (rHuEpo) and (3) saline (control). Both pTarget/EPO and rHuEpo significantly increased (P < 0.05) hematocrit levels 1 week after injection and they remained significantly higher than the control for up to 5 weeks (P < 0.05), showing that both EPO treatments were effective in stimulating the production of red blood cells in rabbits. The EPO gene transfer or rHuEPO administration had no significant effect (P > 0.05) on sperm motility, vigor, viability, concentration or morphology in the testis. |
| Databáze: | Directory of Open Access Journals |
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