| Autor: |
Isabela Neves de Almeida, Agdemir Valéria Aleixo, Wânia da Silva Carvalho, Silvana Spindola de Miranda |
| Jazyk: |
English<br />Spanish; Castilian |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Revista Argentina de Microbiología, Vol 47, Iss 1, Pp 47-49 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
1851-7617 |
| DOI: |
10.1016/j.ram.2014.12.002 |
| Popis: |
The possibility to obtain DNA from smears is a valuable alternative to remedy the lack of samples when they are totally used for bacilloscopy; this technique solves the biosafety problem related to a possible accident with the transportation of flasks containing potentially transmissible clinical samples. Hence, the purpose of this study was to utilize the insertion sequence IS6110 for amplification of DNA from a smear-positive sample for tuberculosis (TB) diagnosis. Among the 52 positive bacilloscopies, sensitivity, specificity, positive predictive value and negative predictive value were 52.3 %, 100 %, 100 % and 89.7 %, respectively whereas accuracy was 90.7 %. The IS6110-based PCR for TB diagnosis developed in DNA extracted from a positive smear is a fast, simple, specific, and safe method. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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