| Autor: |
Sophie Martin, Gwénaële Jégou, Aurore Nicolas, Matthieu Le Gallo, Éric Chevet, Florence Godey, Tony Avril |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 3, Iss 1, Pp 101229- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2022.101229 |
| Popis: |
Summary: This protocol describes a flow cytometry approach to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in COVID-19-positive patient sera samples without the need of specific laboratory facilities for viral infection. We developed a human-cell-based system using spike-expressing HEK293T cells that mimics membrane insertion and N-glycosylation of viral integral membrane proteins in host cells. This assay represents a powerful tool to test antibody responses against SARS-CoV-2 variants and vaccine effectiveness.For complete details on the use and execution of this protocol, please refer to Martin et al. (2021). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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