Peran antibodi kuning telur (Igy) sebagai opsonin untuk pencegahan serangan Mutan streptococcus serotipe d (Streptococcus sobrinus)
| Autor: | Okti Nadia Poetri, Retno D. Soejoedono, Agustin Indrawati, I Wayan T. Wibawan |
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| Jazyk: | English<br />Indonesian |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Journal of Biological Researches, Vol 13, Iss 2, Pp 129-134 (2012) |
| Druh dokumentu: | article |
| ISSN: | 0852-6834 2337-389X |
| DOI: | 10.23869/bphjbr.13.2.20085 |
| Popis: | The aim of this study was to explore the role of serotype d Mutan Streptococcus (Streptococcus sobrinus) spesific immunoglobulinY (IgY-Ss) as opsonin against the same strain. The eggs were collected from Single Comb Brown Leghorn which has been immunizedwith Streptococcus sobrinus. Agar gel precipitation test was applied to detect IgY-Ss in serum and egg. Egg containing IgY-Ss wascollected and extracted by PEG-Amonium sulphate and purified using fast protein liquid chromatography. The purity of IgY-Ss wasdetermined by UV spectrophotometer. Molecular weight was established by SDS-PAGE (sodium dedocyl sulphate-poly acrilamide gelelectrophoresis). Biological activities of IgY-Ss as opsonin was determined by phagocytosis assay. Phagositic activity of macrophageswas not increased by preincubation of both S. sobrinus (107 CFU/ml) and 100 μg of IgY-S, however the phagositic capacity wasincreased from 1.6 bacterial cell/ macrophag to 5.17 bacterial cell/ macrophag. These finding suggest that IgY-Ss obtained from hensimmunized with S. sobrinus provide an alternative to prevent S. sobrinus infection. |
| Databáze: | Directory of Open Access Journals |
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