| Autor: |
Qiang Huang, Yu-Jie Shen, Chi-Yao Hsueh, Yi-Fan Zhang, Xiao-Hui Yuan, Yu-Juan Zhou, Jiao-Yu Li, Lan Lin, Chun-Ping Wu, Chun-Yan Hu |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Pathology and Oncology Research, Vol 28 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1532-2807 |
| DOI: |
10.3389/pore.2022.1610699 |
| Popis: |
Purpose: This study aimed to investigate the applicability of plasma extracellular vesicles (EVs) miR-99a-5p as a potential head and neck squamous cell carcinoma (HNSCC) diagnostic biomarker.Methods: The miRNA expression of HNSCC tissue and plasma EVs were profiled by small RNA sequencing. qRT-PCR was performed to detect miR-99a-5p expression in HNSCC (n = 93) and benign disease (n = 39) plasma EVs and formalin-fixed and paraffin-embedded (FFPE) tissue (n = 110). We constructed receiver-operating characteristic curves to investigate the diagnostic efficiency of plasma EVs miR-99a-5p.Results: Tumor tissue exhibited lower miR-99a-5p than para-tumor tissue. Patients with high miR-99a-5p expression exhibited significantly more p16 positive status. In contrast, HNSCC plasma EVs harbored more miR-99a-5p than the benign disease group. Plasma EVs miR-99a-5p distinguished HNSCC with area under the curve (AUC) of 0.7494 (95% CI: 0.6692–0.8296; p < 0.0001), with 61.54% sensitivity and 75.27% specificity, respectively. Furthermore, plasma EVs miR-99a-5p also distinguished early HNSCC with AUC of 0.7394 (95% CI: 0.6284–0.8504; p = 0.0002), with 79.07% sensitivity and 61.54% specificity, respectively.Conclusion: Plasma EVs miR-99a-5p is a potential biomarker for predicting early HNSCC. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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