| Autor: |
Ivan Aranha, Yeldur P. Venkatesh |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Journal of Ayurveda and Integrative Medicine, Vol 11, Iss 2, Pp 140-146 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
0975-9476 |
| DOI: |
10.1016/j.jaim.2017.10.006 |
| Popis: |
Background: In traditional medicine, guduchi (Tinospora cordifolia) is considered as an adaptogen with immunomodulatory prowess. A 25 kDa protein from guduchi stem has been characterized as an immunomodulatory protein (ImP). Objectives: The aim of this study was to evaluate the intrinsic immunogenicity of guduchi ImP and adjuvant activity using ovalbumin (OVA) as antigen in BALB/c mice. Materials and Methods: Mice were given guduchi ImP (30 and 60 μg) by intranasal administration to respective groups (n = 6) on days 1, 14 and thereafter weekly till day 42. Immunogenic response was monitored by serum IgG/IgA levels (days 14, 35 and 50). The adjuvant activity was measured by serum anti-OVA IgG/IgA responses to administration of 30 μg OVA with guduchi ImP. The effect of guduchi ImP on the spleen status was examined by splenic weight (day 50). Results: Guduchi ImP administration displayed a significant increase in anti-guduchi ImP IgG (5–7 fold) and anti-guduchi ImP IgA (3–4 fold) on day 50 vs. control. Guduchi ImP showed a significant increase in anti-OVA IgG (6–7 fold) and anti-OVA IgA (4–5 fold) on day 50 vs. control. The splenic index of guduchi ImP group increased significantly in both the immune and adjuvant response groups; however, the splenic index in the adjuvant response group was markedly higher. Conclusion: The results indicate that guduchi ImP is a strong immunogen by itself and enhances the immunogenicity of mucosally-administered antigen in BALB/c mice. Based on the results of this animal study, it appears that guduchi ImP shows a potential for future studies in humans. |
| Databáze: |
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| Externí odkaz: |
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