A novel laparoscopic near-infrared fluorescence spectrum system with indocyanine green fluorescence overcomes limitations of near-infrared fluorescence image-guided surgery

Autor: Yuma Ebihara, Liming Li, Takehiro Noji, Yo Kurashima, Soichi Murakami, Toshiaki Shichinohe, Satoshi Hirano
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Journal of Minimal Access Surgery, Vol 18, Iss 1, Pp 125-128 (2022)
Druh dokumentu: article
ISSN: 0972-9941
1998-3921
DOI: 10.4103/jmas.JMAS_165_20
Popis: Background: Near-infrared (NIR) fluorescence image-guided surgery (FIGS) introduces a revolutionary new approach to address this basic challenge in minimally invasive surgery. However, current FIGS systems have some limitations – the infrared rays cannot detect and visualise thick tissues with low concentrations of the fluorescent agent. We established a novel laparoscopic fluorescence spectrum (LFS) system using indocyanine green (ICG) fluorescence to overcome these limitations. Materials and Methods: Bovine serum albumin (BSA) was conjugated to ICG, and the mixtures were serially diluted at 5 × 10−8–5 × 10−1 mg/mL. We used the LFS system and a NIR camera system (NLS; SHINKO OPTICAL CO., LTD Tokyo, Japan) to determine the optical dilution for the fluorescence detection. BSA was conjugated to ICG (5.0 × 10−2 mg/mL) and used to coat the clips. We attempted to identify the fluorescence-coated clip from the serosal side of the cadaveric porcine stomach tissues using the LFS system and the NIR camera system. We measured the depth of the cadaveric porcine stomach wall at the thickest part that could be confirmed. Results: We could not visualise fluorescence concentrations
Databáze: Directory of Open Access Journals
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