| Autor: |
Clemow Scott R, Clairmont Lindsey, Madsen Lene H, Guinel Frédérique C |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Plant Methods, Vol 7, Iss 1, p 46 (2011) |
| Druh dokumentu: |
article |
| ISSN: |
1746-4811 |
| DOI: |
10.1186/1746-4811-7-46 |
| Popis: |
Abstract Pea has lagged behind other model legumes in the molecular study of nodulation and mycorrhizae-formation because of the difficulty to transform its roots and its poor growth on agar plates. Here we describe for pea 1) a transformation technique which permits the complementation of two known non-nodulating pea mutants, 2) a rhizobial inoculation method which allows the study of early cellular events giving rise to nodule primordia, and 3) a targeted fungal inoculation method which allows us to study short segments of mycorrhizal roots assured to be infected. These tools are certain to advance our knowledge of pea root symbioses. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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