| Autor: |
Juan Pablo Arias, Karol Zapata, Benjamín Rojano, Mariana Peñuela, Mario Arias |
| Jazyk: |
Spanish; Castilian |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Revista U.D.C.A Actualidad & Divulgación Científica, Vol 20, Iss 2, Pp 353-362 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
0123-4226 |
| Popis: |
Cell suspension cultures of Thevetia peruviana were established under dark for 19 days to investigate kinetic behavior related to biomass, substrate, cardiac glycoside, polyphenols, reactive oxygen species and anti-oxidant activity. The results showed high biomass production (18.80gDW/L) as well as sucrose consumption in 7 days. Preferential glucose over fructose consumption was observed. Intracellular production of cardiac glycosides reached 2.58mg DE/gDW at day 19. Highest extracellular production was reached between day 2 and 7 (6.19mg DE/L). Highest extracellular phenolic content was 80.61 ± 5.16mg GAE/L at day 7. Intracellular phenolic content increased to 2.76 ± 0.14mg GAE/gFW at day 7 and remained constant until day 19. ROS production at day 7 could be related to sucrose and glucose total consumption. Pearson Product-Moment Correlation Coefficient (ρ) showed that the phenolic compounds in cell suspension cultures of T. peruviana were responsible for the observed anti-oxidant activity. All together, these results give the first steps in metabolic behavior in cell suspension cultures of T. peruviana. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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