Isolation and characterization of antagonistic Paenibacillus polymyxa HX-140 and its biocontrol potential against Fusarium wilt of cucumber seedlings

Autor: Yang Zhai, Jiu-xiang Zhu, Tai-meng Tan, Jian-ping Xu, Ai-rong Shen, Xie-bin Yang, Ji-lie Li, Liang-bin Zeng, Lin Wei
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: BMC Microbiology, Vol 21, Iss 1, Pp 1-12 (2021)
Druh dokumentu: article
ISSN: 1471-2180
03490483
DOI: 10.1186/s12866-021-02131-3
Popis: Abstract Objective The aim of this study is to evaluate the efficacy of the strain Paenibacillus polymyxa HX-140, isolated from the rhizosphere soil of rape, to control Fusarium wilt of cucumber seedlings caused by Fusarium oxysporum f. sp. cucumerinum. Results Strain HX-140 was able to produce protease, cellulase, β-1,3-glucanase and antifungal volatile organic compounds. An in vitro dual culture test showed that strain HX-140 exhibited broad spectrum antifungal activity against soil-borne plant pathogenic fungi. Strain HX-140 also reduced the infection of Fusarium wilt of cucumber seedlings by 55.6% in a greenhouse pot experiment. A field plot experiment confirmed the biocontrol effects and further revealed that antifungal activity was positively correlated with inoculum size by the root-irrigation method. Here, inoculums at 106 107 and 108 cfu/mL of HX-140 bacterial suspension reduced the incidence of Fusarium wilt of cucumber seedling by 19.5, 41.1, and 50.9%, respectively. Conclusions Taken together, our results suggest that P. polymyxa HX-140 has significant potential in the control of Fusarium wilt and possibly other fungal diseases of cucumber.
Databáze: Directory of Open Access Journals
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