Mutational and acquired carbapenem resistance mechanisms in multidrug resistant Pseudomonas aeruginosa clinical isolates from Recife, Brazil

Autor: Felipe Lira de Sá Cavalcanti, Cristina Rodríguez Mirones, Elena Román Paucar, Laura Álvarez Montes, Tereza Cristina Leal-Balbino, Marcia Maria Camargo de Morais, Luis Martínez-Martínez, Alain Antonio Ocampo-Sosa
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Memorias do Instituto Oswaldo Cruz, Vol 110, Iss 8, Pp 1003-1009 (2015)
Druh dokumentu: article
ISSN: 1678-8060
0074-0276
DOI: 10.1590/0074-02760150233
Popis: An investigation was carried out into the genetic mechanisms responsible for multidrug resistance in nine carbapenem-resistant Pseudomonas aeruginosaisolates from different hospitals in Recife, Brazil. Susceptibility to antimicrobial agents was determined by broth microdilution. Polymerase chain reaction (PCR) was employed to detect the presence of genes encoding β-lactamases, aminoglycoside-modifying enzymes (AMEs), 16S rRNA methylases, integron-related genes and OprD. Expression of genes coding for efflux pumps and AmpC cephalosporinase were assessed by quantitative PCR. The outer membrane proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The blaSPM-1, blaKPC-2 and blaGES-1 genes were detected in P. aeruginosaisolates in addition to different AME genes. The loss of OprD in nine isolates was mainly due to frameshift mutations, premature stop codons and point mutations. An association of loss of OprD with the overexpression of MexAB-OprM and MexXY-OprM was observed in most isolates. Hyper-production of AmpC was also observed in three isolates. Clonal relationship of the isolates was determined by repetitive element palindromic-PCR and multilocus sequence typing. Our results show that the loss of OprD along with overexpression of efflux pumps and β-lactamase production were responsible for the multidrug resistance in the isolates analysed.
Databáze: Directory of Open Access Journals
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