| Autor: |
Benchaporn Lertanantawong, Abhichart Krissanaprasit, Thanyarat Chaibun, Kurt Vesterager Gothelf, Werasak Surareungchai |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Materials Science for Energy Technologies, Vol 2, Iss 3, Pp 503-508 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2589-2991 |
| DOI: |
10.1016/j.mset.2019.05.001 |
| Popis: |
One of the great challenges for the development of biosensors is to construct a fast, practical and multiplexed detection platform. One promising approach is utilizing the controllable conformation of DNA nanostructures for such biosensing applications. Here, we present tweezer-like DNA (TW-DNA)-assisted multiplexed DNA detection in a one-pot reaction based on fluorescence quenching. The open conformation of the TW-DNA structure can be changed to a closed state by a specific single-stranded DNA target. The fluorophore/quencher pairs decorated on the TW-DNA and the conformation changes were observed by fluorescence quenching. In its initial state, the TW-DNA is maintained in an open state that demonstrates high fluorescence intensity. In the presence of a specific target DNA, the TW-DNA changes to a closed conformation and the emission intensity of the donor is quenched which is directly proportional to the target concentration. Three DNA targets were simultaneously detected using three individually self-assembled TW-DNA that were modified appropriately with fluorophore/quencher pairs for the specific detection of the individual DNA target. The detection range of this assay is at submicromolar concentration of multiple DNA targets with a fast detection time of one minute. Moreover, this biosensor platform provides the significant advantage of being a one-pot reaction for multiplexed DNA detection without any cross-talk, thereby making it a highly applicable assay. Keywords: Multiplexing, DNA detection, Fluorescence quenching, One-pot detection, DNA nanostructure |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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