Biocidal effects of a wipe-down procedure using common veterinary cleansers on microbial burden within working canine exterior coats
Autor: | Erin B. Perry, Dakota R. Discepolo, Stephen Y. Liang, Maurnice Scott, Kyleigh Williamson, Kelly S. Bender |
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Jazyk: | angličtina |
Rok vydání: | 2023 |
Předmět: | |
Zdroj: | Frontiers in Veterinary Science, Vol 10 (2023) |
Druh dokumentu: | article |
ISSN: | 2297-1769 18830056 |
DOI: | 10.3389/fvets.2023.1219249 |
Popis: | IntroductionRecent work demonstrating reduction of aerosolized contamination via a wipe-down procedure using common veterinary antiseptics offers promise regarding health concerns associated with cross-contamination from working canines to humans. While mechanical reduction can be achieved via a wipe-down procedure, the biocidal impact on flora within the exterior coat is unknown.MethodologyThis study assessed the biocidal impact of antiseptics on the exterior bacterial community of the canine. Lint-free towels were saturated with 2% chlorhexidine gluconate scrub, or 7.5% povidone-iodine scrub diluted at a 1:4 ratio. Treatments were rotated across the dorsal aspect of kennel housed Foxhounds (n = 30). Sterile swabs were collected in triplicate prior to, and following wipe down, stored in Amies solution at 4°C, plated onto nutrient agar and reduction in colony forming units (CFU) was measured across both treatments. Statistical analysis utilizing PROC GLM examined effects of treatment (p ≤ 0.05). Molecular analysis of the 16S rRNA gene was completed for 3 hounds.ResultsReduction in CFU was measured (p |
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