| Autor: |
Mohammed El Behery, Mika Fujimura, Tetsunari Kimura, Motonari Tsubaki |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Biochemistry and Biophysics Reports, Vol 21, Iss , Pp - (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2405-5808 |
| DOI: |
10.1016/j.bbrep.2020.100730 |
| Popis: |
We studied human 101F6 protein to clarify its physiological function as a ferric reductase and its relationship to tumor suppression activity. We found for the first time that purified 101F6 both in detergent micelle state and in phospholipid bilayer nanodisc state has an authentic ferric reductase activity by single turnover kinetic analyses. The kinetic analysis on the ferrous heme oxidation of reduced 101F6 upon the addition of a ferric substrate, ferric ammonium citrate (FAC), showed concentration-dependent accelerations of its reaction with reasonable values of KM and Vmax. We further verified the authenticity of the ferric reductase activity of 101F6 using nitroso-PSAP as a Fe2+-specific colorimetric chelator. 101F6 in nanodisc state showed higher efficiency for FAC than in detergent micelle state. Keywords: Human 101F6 protein, Cytochrome b561, Ferric reductase activity, Nanodisc reconstitution, FAC, Nitroso-PSAP |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
