TaqMan®-based quantification of invasive cells in the chick embryo metastasis assay
| Autor: | Edward Htun van der Horst, Jörg H. Leupold, Rainer Schubbert, Axel Ullrich, Heike Allgayer |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 37, Iss 6, Pp 940-946 (2004) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/04376ST02 |
| Popis: | The chick embryonic metastasis (CEM) assay is a fast in vivo method to investigate the invasive properties of tumor cells. Until now, most quantification methods were semiquantitative and time-consuming. Here we describe a rapid quantification method using TaqMan® technology to quantify the invaded tumor cells in the chorioallantoic membrane of fertilized eggs. This method is based on specific detection of human ALU sequences. Moreover, it provides high sensitivity over a wide linearity range. |
| Databáze: | Directory of Open Access Journals |
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