Frozen Semen Characteristics of Limousin Bull at Different Ages
| Autor: | A. Baharun, A. B. Setiawan, A. Rahmi, H. Iskandar, M. Gunawan, S. Anwar, T. Maulana, E. M. Kaiin, S. Said |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Tropical Animal Science Journal, Vol 46, Iss 3 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2615-787X 2615-790X |
| DOI: | 10.5398/tasj.2023.46.3.306 |
| Popis: | Strategies to increase the population and productivity of beef cattle can be implemented through reproductive management, such as artificial insemination (AI) using frozen semen. This study aims to evaluate the characteristics of frozen semen of Limousin bulls of different ages, which can still be used for insemination programs up to 12 years of age. This study used frozen semen of Limousin bulls ages 3, 8, and 12 years produced in 2021 at the Artificial Insemination Center in Singosari, East Java Province, Indonesia. Five bulls from each different age group were used for replication. Computer-assisted sperm analysis was used to determine sperm motility, viability, and abnormalities using eosin–nigrosine staining. Plasma membrane integrity was analyzed using the hypoosmotic swelling test. The acrosomes’ integrity was evaluated using FITC-PNA-PI, protamine deficiency using chromomycin A3, and DNA fragmentation testing using the acridine orange fluorescent technique. The results showed that the parameters of sperm motility, viability, abnormality, plasma membrane integrity, and protamine deficiency showed no significant differences in all age groups. The kinematic parameters (straightness and beat cross frequency) of the 3-year-old group were significantly higher (p |
| Databáze: | Directory of Open Access Journals |
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