Distinct antibody response in susceptible and non-susceptible hosts of the carcinogenic liver fluke Opisthorchis viverrini infection

Autor: Krongkarn Watakulsin, Arpa Surapaitoon, Lorina Handayani Ulag, Sunheng Kaing, Watcharapol Suyapoh, Prasert Saichua, Kanin Salao, Sirikachorn Tangkawatana, Sutas Suttiprapa
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Parasitology, Vol 150, Pp 653-660 (2023)
Druh dokumentu: article
ISSN: 00311820
0031-1820
1469-8161
DOI: 10.1017/S0031182023000112
Popis: Opisthorchis viverrini is a carcinogenic parasite that can cause bile duct cancer called cholangiocarcinoma. A study of the immune response of this parasite in susceptible and non-susceptible hosts may provide a clue to develop vaccines and immunodiagnostic markers, which are currently not available. Here, we compared the antibody response in susceptible Golden Syrian hamsters and non-susceptible BALB/c mice infected by the liver fluke. In mice, the antibody was detected between 1 and 2 weeks post-infection, whereas it was positive between 2 and 4 weeks post-infection in hamsters. Immunolocalization revealed that the antibody from mice reacts strongly with the tegumental surface and gut epithelium of the worm, while hamster antibody showed a weak signal in the tegument and a comparable signal in the gut of the worm. Immunoblot of the tegumental proteins demonstrated that while hamster antibody showed a broad specificity, mice strongly reacted with a single protein band. Mass spectrometry revealed these immunogenic targets. Recombinant proteins of the reactive targets were produced in the bacterial expression system. The immunoblot of these recombinant proteins confirm the reactivity of their native form. In summary, there is a different antibody response against O. viverrini infection in susceptible and non-susceptible hosts. The non-susceptible host reacts quicker and stronger than the susceptible host.
Databáze: Directory of Open Access Journals
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