Molecular Identification and Biochemical Characterization of Novel Marine Yeast Strains with Potential Application in Industrial Biotechnology

Autor: Boutheina Bessadok, Bassem Jaouadi, Thomas Brück, Andrea Santulli, Concetta Maria Messina, Saloua Sadok
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Fermentation, Vol 8, Iss 10, p 538 (2022)
Druh dokumentu: article
ISSN: 2311-5637
DOI: 10.3390/fermentation8100538
Popis: Cell-based agriculture is an emerging and attractive alternative to produce various food ingredients. In this study, five strains of marine yeast were isolated, molecularly identified and biochemically characterized. Molecular identification was realized by sequencing the DNA ITS1 and D1/D2 region, and sequences were registered in GenBank as Yarrowia lipolytica YlTun15, Rhodotorula mucilaginosa RmTun15, Candida tenuis CtTun15, Debaryomyces hansenii DhTun2015 and Trichosporon asahii TaTun15. Yeasts showed protein content varying from 26% (YlTun15) to 40% (CtTun15 and DhTun2015), and essential amino acids ranging from 38.1 to 64.4% of the total AAs (CtTun15-YlTun15, respectively). Lipid content varied from 11.15 to 37.57% with substantial amount of PUFA (>12% in RmTun15). All species had low levels of Na (
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