| Autor: |
Joong-Hwan Lee, Chang-Gi Son, Joong-Bae Kwon, Hyo-Hun Nam, Yeong-Tae Kim, Bong-Choon Lee, Dong-Bum Shin |
| Jazyk: |
English<br />Korean |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Research in Plant Disease, Vol 23, Iss 1, Pp 65-68 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1598-2262 |
| DOI: |
10.5423/RPD.2017.23.1.65 |
| Popis: |
Barley yellow mosaic virus (BaYMV), which is transmitted by the root-inhabiting protist Polymyxa graminis, causes a soil-borne disease. In this study, we detected BaYMV from soil using two-step nested polymerase chain reaction (PCR). Specific primers based on a coat protein region of BaYMV segment RNA1 were used in the first round of amplification. Based on the sequenced amplicon, an inner primer was designed for the second round of amplification. A PCR product of 372 bp exhibited 98%–100% nucleotide sequence identity with the coat protein region of BaYMV segment RNA1. In this study, we propose an easy method for the detection of BaYMV from soil, may considerably assist in accurate fungus-transmitted virus diagnosis and subsequent disease forecasting. This is the first report on the detection of BaYMV from soil. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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